Zetter guest Ed. JAI Press Inc. Cohen S , Langer, R. Puisieux, P. Couvreur et al. Editions de Sante', Paris pp. Biocompatible alginate scaffolds enabling prolonged hepatocyte functions in culture. Tissue Engineering for Therapeutic Use 3, Y. Ikada and T. Okano, Eds. Dvir T, Cohen S. Langer , Artech House, pp.
Citations per year
In: Myocardial Tissue Engineering Ed. Amit Gefen , Springer-Verlag. Ruvinov E, Cohen S. Re'em T, Cohen S. Springer Publishing Company, pp. Gaharwar, S. Sant, M. Hancock, S. In: A. Even if iPSCs have the potential to be considered the best solution to repopulate an acellular cardiac scaffold, some issues still need to be resolved: improving their effectiveness of dedifferentiation, removing the risk of teratoma development, improving culture techniques, and enhancing new strategies for their distribution into acellular scaffolds [ 97 ].
Finally, growth factor addition has to be taken into consideration for efficient recellularization. Many growth factors can be used in cardiac bioengineering, and the most significant are bone morphogenetic protein BMP [ ], basic fibroblast growth factor bFGF [ ], BMP-4 [ 93 ], and vascular endothelial growth factor VEGF [ ].
In , Van Wijk and colleagues [ ] summarized how BMPs are crucial for cardiac differentiation and for dedifferentiation starting from iPSCs not only in specific heart-forming regions but also at cardiac distal margins. Perets and colleagues [ ] demonstrated how bFGF could stimulate angiogenesis inducing the proliferation of endothelial cells, smooth muscle cells, and fibroblast on alginate scaffolds.
VEGF has been identified as one of the major stimuli for angiogenesis in vitro and in vivo that actually remains a big challenge to address limiting organ bioengineering. After implantation, VEGF-containing matrix was adapted into native vascularized tissue. In recent years, several bioengineered tissues have been created and transplanted in humans. These were relatively simple structures such as blood vessels, upper airway tubes, or urogenital tissues. The larger challenge, however, remains the bioengineering of complex parenchymal organs for example, the kidney or liver for human transplantation.
Even though this number of organs represents only about However, a major challenge still exists in the complete repopulation of these whole-organ scaffolds, which is necessary to produce a clinically functional organ. Identification of a cell source that has the potential to proliferate after scaffold seeding may offer a solution. Furthermore, even if the whole-organ ECM scaffold was made from animal tissue, their species-specific biological and biomechanical properties are suitable for human cell seeding.
Lastly, the use of discarded human organs, with a complete patient history, can facilitate regulatory approval of these scaffolds for clinical use. They provide a natural environment for seeded cells, similar to the native organ, and include organ-specific biochemical stimuli such as growth factors, cytokines, or chemokines. They maintain the original 3D architecture after decellularization. They can be transplanted in vivo via a dual vascular pedicle arterial and venous , guaranteeing physiological oxygen and nutrient supply.
These include the determination of specific criteria for successful decellularization, identification of a reliable cell source for the recellularization, and the development of models for bioengineered organ transplantation with long-term follow-up studies that can translate into clinical practice. World Health Organization. World Health Statistic. Geneva, Switzerland: World Health Organization; United Network for Organ Sharing.
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Accessed April Atala A. Engineering tissues, organs and cells. J Tissue Eng Regen Med. Organ bioengineering and regeneration as the new Holy Grail for organ for organ transplantation. Ann Surg. An overview of tissue and whole organ decellularization processes.
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Degradation products of extracellular matrix affect cell migration and proliferation. Tissue Eng Part A. Comparison of three methods for the derivation of a biologic scaffold composed of adipose tissue extracellular matrix. Tissue Eng Part C. Repopulation of decellularized whole organ scaffold using stem cells: an emerging technology for the development of neo-organ. J Artif Organs. The use of whole organ decellularization for the generation of vascularized liver organoid.
Hynes RO. The extracellular matrix: not just pretty fibrils.
Role of the extracellular matrix in regulating stem cell fate. Nat Rev Mol Cell Biol. The extracellular matrix: a dynamic niche in cancer progression. J Cell Biol. Balancing forces: architectural control of mechanotransduction. Extracellular matrix: a dynamic microenvironment for stem cell niche. Biochim Biophys Acta. Perspective on whole-organ assembly: moving toward transplantation on demand. J Clin Invest. Higgens G, Anderson R.
Methods in bioengineering : 3D tissue engineering in SearchWorks catalog
Experimental pathology of the liver: restoration of liver of the white rat following partial surgical removal. Arch Pathol Lab Med. World Health Statistics.
Chronic Liver Disease and Cirrhosis. Accessed 25 Jun Waiting List Candidates. Nat Med. Badylak SF.
Publikationen Prof. Dr. Eric Gottwald
The extracellular matrix as a biologic scaffold material. Biomaterials for tissue engineering. World J Urol. Assessing porcine liver-derived biomatrix for hepatic tissue engineering. Tissue Eng. Human-scale whole-organ bioengineering for liver transplantation: a regenerative medicine approach. Cell Transplant. Bioengineered transplantable porcine livers with re-endothelialized vasculature.
Method for the decellularization of intact rat liver. Liver-derived extracellular matrix as a biologic scaffold for acute vocal fold repair in a canine model. Determining the optimal decellularization and sterilization protocol for preparing a tissue scaffold of a human-sized liver tissue. Induced pluripotent stem cell-derived hepatocytes have the functional and proliferative capabilities needed for liver regeneration in mice.
Toward an extended functional hepatocyte in vitro culture. Tissue Eng Part C Methods.
Related Methods in Bioengineering: 3d Tissue Engineering (The Artech House Methods in Bioengineering)
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